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A computer-based data acquisition and analysis system was developed customized to EMG measurement and analysis requirements. The system was designed as an assistive tool for researchers studying human electrophysiology and kinesiology. A user-friendly interface characterized by a great adaptability and compatibility with other similar existing software packages was implemented, enabling multichannel acquisition, data storage and analysis. The application integrates the most frequent processing techniques required by EMG researchers and analysts, as well as a number of advanced signal processing techniques adjusted to the features of the EMG signal. The system comprises flexible signal analysis and display modules, forming a fully operational tool that can be easily configured to serve any similar purposed measurement requirements.