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Cucurbiturils: molecular nanocapsules for time-resolved fluorescence-based assays

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3 Author(s)
Marquez, C. ; Sch. of Eng. & Sci., Int. Univ. Bremen, Germany ; Fang Huang ; Nau, W.M.

A new fluorescent host-guest system based on the inclusion of the fluorophore 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) into the cavity of the molecular container compound cucurbit[7]uril (CB7) has been designed which possesses an exceedingly long-lived emission (690 ns in aerated water). The large binding constant of (4±1)×105 M-1 along with the resistance of the CB7·DBO complex toward external fluorescence quenchers allow the use of CB7 as an enhancer in time-resolved fluorescence-based assays, e.g., to screen enzyme activity or inhibition by using DBO-labeled peptides as substrates. The response of CB7·DBO to different environmental conditions and possible quenchers are described.

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NanoBioscience, IEEE Transactions on  (Volume:3 ,  Issue: 1 )