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The generation of optical frequency combs, directly referenced to the SI second, can be used to make measurements of optical frequencies. This provides a supreme method for the realization of the meter. However, an approximate knowledge of the frequency of the radiation is normally needed for such measurements in order to determine the integral order of the comb component used. Such information is usually obtained by prior wavelength measurements of the radiation under study. This paper demonstrates a new method to determine the absolute mode number in optical frequency measurements using mode-locked femtosecond lasers, thus eliminating the need for complementary wavelength measurements. Measurements of the frequency of an iodine-stabilized He-Ne laser at λ=633 nm and a Nd:YAG laser at λ=532nm are given as examples.