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ACAPELLA-5K, a high-throughput automated genome and chemical analysis system

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9 Author(s)
Meldrum, D.R. ; Dept. of Electr. Eng., Washington Univ., Seattle, WA, USA ; Fisher, C.H. ; Moore, M.P. ; Saini, M.
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A capillary-based fluid handling system bas been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-μL final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on.

Published in:

Intelligent Robots and Systems, 2003. (IROS 2003). Proceedings. 2003 IEEE/RSJ International Conference on  (Volume:3 )

Date of Conference:

27-31 Oct. 2003