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In this paper, we investigate the use of layered space-time (also known as the vertical Bell Laboratories layered space-time (V-BLAST) scheme) for multiuser detection in fading channels. The multiple transmit antennas in V-BLAST are treated as individual mobile station transmitters, while the base station consists of multiple receive antennas. In the proposed system, users are organized in groups and allocated a unique spreading code within the same group. Using these codes, we are able to separate the different groups, and layered space-time algorithm is then invoked to further remove the remaining interference between users. A decorrelator-type receiver-based layered space-time detection is proposed for both complex and real constellations. For the latter case, we derive our receiver after evaluating and comparing the performance of two decorrelators based on the V-BLAST scheme. It is demonstrated that a significant performance improvement and increase in system capacity is obtained with very low spreading factors. Further results are also introduced by considering reduced complexity receivers based on serial layered space-time group multiuser detection, and parallel layered space-time group multiuser detection.