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This letter addresses the optimum energy partition between the midamble and data fields, given the total energy per burst, in channel estimation for joint detected time-division code-division multiple-access systems. We show that, given the lengths of the data and midamble, in general, the optimal solution requires different amplitude levels. We also derive the burst structure leading to the optimum energy partition with equal amplitude symbols, and the performance degradation by using traditional choices. The analysis is validated by simulations.