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An efficient electrochemical detector using 3-D comb electrodes was proposed and fabricated by simple micro fabrication technology. The dimensions of fabricated 3-D electrodes were 10 /spl mu/m in width, 30 /spl mu/m in height, and 10 /spl mu/m gap. A prototype of micro flow cell for electrochemical immunoassay using 3-D electrodes was designed and fabricated. The qualitative evaluation of the flow cell for enzyme immunoassay will be discussed.