By Topic

Intact extracellular matrix can be utilized for bio-integrated materials and tissue engineering

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

7 Author(s)
Yamashita, K. ; Dept. of Anatomy, Tokushima Univ., Japan ; Morimoto, H. ; Nakao, M. ; Ukai, H.
more authors

Although the organic macromolecules gelatin, collagen and pure non-collagenous protein have been utilized as biomaterials, intact extracellular matrix without cells has not yet been utilized. In order to investigate the possibility of using intact extracellular matrix as a novel biomaterial with bone-inductive activity, we analyzed the structure formed by cultured clonal MC3T3-E1 osteoblasts on the calcified surface of commercial pure titanium, after removal of the cells from the surface of the titanium. We found that extracellular matrix collagen fibers with attached non-collagenous protein grew at the surface of titanium, and that they were attached to calcified areas of titanium via a structure we called "anchor structure". Here, we show not only that a biomaterial consisting of titanium with attached intact extracellular matrix can be developed, but also that intact extracellular matrix can be extracted without osteoblasts for use in medical treatments. Furthermore, titanium with attached intact extracellular matrix could be applied to culture systems (by virtue of its tissue-inducing activity) and tissue engineering.

Published in:

Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference on

Date of Conference: