By Topic

Microchip-based techniques with benefits for single cell characterization using optical analyses systems

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
H. Thielecke ; Dept. of Biohybrid Syst., Fraunhofer Inst. for Biomed. Eng., St. Ingbert, Germany ; A. A. Robitzki

In medical biotechnology there is a growing need for efficient and reliable single cell analysis techniques. Fluorescence microscopy in combination with image analysis solution is a powerful tool for the characterization of cells. The performance and reliability of image analysis for cell characterization can be much improved if the cells are arranged in array structures. Here we introduce a microchip based approach for the analysis on the single cell level by using fluorescence techniques. pEGFP transfected and diacetyl-fluorescein treated embryonic rat oligodendrocytes; (OLN-93) are arranged in array structures by hydrodynamic positioning onto pores of microfabricated chips. Fluorescence micrographs of separated single cells in array structures are obtained. Different fluorescence intensities of single pEGFP-transfected oligodendrocytes; are distinguishable. Using the proposed technique cells can be identified which fulfill predetermined criteria and easily addressed in the array. Further, the use of electrical impedance spectroscopy for positioning control and for determining the viability of the cells is discussed.

Published in:

Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference on

Date of Conference: