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Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes

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3 Author(s)
J. R. Buitenweg ; Signals & Syst. Group, Univ. of Twente, Enschede, Netherlands ; W. L. C. Rutten ; E. Marani

Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of voltage-sensitive channels over the neuronal membrane. In this paper, finite-element modeling is used to quantify the effect of these channel distributions on the neuron-electrode contact. Slight accumulation or depletion of voltage-sensitive channels in the sealing membrane of the neuron results in various shapes and amplitudes of simulated extracellular recordings. However, estimation of channel-specific accumulation factors from extracellular recordings can be obstructed by co-occuring ion currents and defect sealing. Experimental data from cultured neuron-electrode interfaces suggest depletion of sodium channels and accumulation of potassium channels.

Published in:

IEEE Transactions on Biomedical Engineering  (Volume:49 ,  Issue: 12 )