By Topic

Designing scaffolds for valvular interstitial cells

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
K. S. Masters ; Howard Hughes Med. Inst., Boulder, CO, USA ; D. N. Shah ; K. A. Davis ; K. S. Anseth

Valvular interstitial cells (VICs) were isolated from porcine aortic heart valves and cultured in vitro on a variety of natural and synthetic surfaces to identify suitable scaffolds for tissue engineering a heart valve. VICs possess many properties that make them attractive for use in the construction of a tissue engineered valve; however, the surfaces to which VICs will adhere and spread are very limited. For example, VICs adhere and spread on collagen and laminin-coated surfaces, but display greatly altered morphology and do not, proliferate. Interestingly, fibronectin was one adhesion protein that facilitated VIC adhesion and proliferation. Yet, VICs did not spread on surfaces modified with RGD, a ubiquitous cell-adhesive peptide, nor to EILDV, a fibronectin-specific peptide sequence. Hyaluronic acid (HA), a highly elastic polysaccharide, was modified to form photopolymerizable hydrogels. VICs were found to spread and proliferate on these gels, forming a confluent monolayer on the gels within four days. Because HA alone experiences rapid enzymatic degradation, it was also combined with photopolymerizable poly(ethylene glycol) (PEG) to form gels with better mechanical properties and extended degradation times. These HA-PEG hydrogels possess desirable macroscopic properties while simultaneously providing a suitable cellular environment for VICs to form a tissue engineered heart valve.

Published in:

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint  (Volume:1 )

Date of Conference:

2002