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Endothelial cells are constantly subjected to mechanical forces due to blood flow. The forces have been implicated in vascular pathogenesis; therefore, the consequences of these forces need to be examined. Using cDNA microarray technology, we studied the gene expression of human umbilical vein endothelial cells subjected to a 10% cyclic strain for 6 and 24 hours. Of the 4000 genes included on the Research Genetics GeneFilter® 211, thirty genes were determined as differentially expressed. The most highly upregulated gene at 6 hours was carboxypeptidase A3 at 3.2-fold; whereas, the most downregulated gene was dystrobrevin, alpha at 0.4-fold. At 24 hours, the most highly upregulated was matrix metalloproteinase 10 at 3.6-fold and the most downregulated was secretory leukocyte protease inhibitor at 0.5-fold. These genes were not only regulated by the strain of the cells, but some were actually regulated by the fluid agitation inherent in the system over the cells.