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A sharp increase in shear stress followed by steady laminar shear stress (step flow) imposed on the surface of macrovascular endothelial cells (ECs) causes changes to the metabolism, gene expression, protein synthesis, as well as to the reactive oxygen species (ROS) production. In this study, we monitored the shear-induced cytosolic ROS production in human umbilical vein ECs (HUVECs) and in human dermal microvascular ECs (HMVECs) using the oxidant-sensitive fluorescent probe 2,7-dichlorodihydrofluorescein diacetate (DCF-DA). HMVECs increased their ROS levels in response to shear stress in a time- and dose-dependent manner, when the shear stress was in the range of those encountered in postcapillary venules (2 or 4 dynes/cm2). whereas HUVECs responded only to a higher shear stress tested (10 dynes/cm2). It is known that many oxidants induce DCF-DA oxidation (hydra peroxides, ONOO-, NO2 radical). In agreement to that, either an ecNOS inhibitor or a ONOO scavenger/SOD mimetic inhibited the shear-induced DCF signal. We are currently establishing a method to monitor lipid peroxidation (formation of lipid hydroperoxides) in the membranes of sheared ECs. Our plan is to investigate the contribution of cytosolic ROS to membranous lipid peroxidation and to intracellular shear-induced signaling.