By Topic

Delivery of PEI-condensed DNA via a porous polymer scaffold for tissue engineering applications

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Y. Huang ; Dept. of Biomed. Eng., Michigan Univ., Ann Arbor, MI, USA ; D. J. Mooney ; K. G. Rice

An attractive approach to tissue engineering is the delivery of therapeutic plasmid DNA through biodegradable polymers. However, the low transfection efficiency of naked DNA might be a limitation. Poly(ethylenimine) (PEI) has been shown to be highly effective as a non-viral gene delivery vehicle and we hypothesized that local delivery of condensed DNA in a three dimensional biodegradable scaffold will enhance transfection efficiency compared to that of uncondensed DNA. To address this, we optimized the characteristics of PEI-DNA condensates encapsulated within PGLA sponge formed by a gas foaming process and performed in vitro transfection studies. Release of uncondensed DNA from sponges was rapid, while release was significantly slower when condensed DNA was incorporated. In vitro transfection was observed only in sponges incorporating condensed DNA, while no transfection was observed for sponges incorporating uncondensed DNA. This study demonstrates the feasibility of incorporating freeze-dried PEI-DNA condensates within PLGA sponges using sucrose as the pore forming agent to efficiently transfect cells, and may find application in areas such as bone tissue engineering.

Published in:

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint  (Volume:1 )

Date of Conference: