By Topic

Simultaneous neurite elicitation and elongation from neurons using a microfabricated post array

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
M. N. De Silva ; Dept. of Chem. Eng. & Mater. Sci., Minnesota Univ., Minneapolis, MN, USA ; A. Baldi ; J. N. Fass ; B. Ziaie
more authors

We report the use of a microfabricated post array with supporting pillars to apply direct mechanical force to initiate and elongate multiple neurites from chick forebrain neurons in vitro. The micropost array was, placed on top of the cell culture on a glass cover slip; a gap of 3-5 μm was achieved between the tips of the posts and the cell culture surface substrate due to the differential height of the posts and the supporting pillars. After allowing ∼1.5 hours for the cells to adhere to the posts, the post array was moved laterally with respect to the substrate and cells at a constant velocity of 36 μm/hr. Several neurite-like cytoplasmic processes initiated from cells that had formed adhesion to the posts, and these neurites elongated in response to further translation. This is the first report of the use of a microfabricated device to simultaneously apply mechanical force to multiple neurons to elicit and elongate neurites. We envision that this technique will enable the construction of living neural networks having defined connectivity.

Published in:

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint  (Volume:2 )

Date of Conference: