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In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophilic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed to fabricate the biochips. The developed technique uses butanol solvent to keep paraffin liquid during spin-coating and thick photoresist for patterning. Using this approach, paraffin layers of 5-70 μm have been successfully fabricated and patterned. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint (Volume:2 )
Date of Conference: 2002