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With the advent of whole genome sequencing, it is now possible to get a complete picture of the cellular activities of an organism. One important tool in the era of functional genomics is the examination of the transcript expression patterns of the entire genome using deoxribonucleic acid microarray technology. This transcriptome analysis, along with proteome analysis, can provide important information regarding the physiological status of a cell under a specific set of experimental conditions. We have examined the transcriptome profile of a brewery strain of yeast and compared it with that of a laboratory strain, under identical brewing conditions. By comparing the global gene expression profiles, we identify genes that are uniquely expressed by the brewery strain. The datasets were also compared to others in the public domain in an effort to understand the precise environmental conditions controlling the gene expression patterns.