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Summary form only given. In this study, intraoperative imaging was performed to investigate the feasibility of OCT for imaging prostate pathology in vivo as well as to investigate OCT intraoperative imaging in a more general context. A sterilizable hand-held surgical probe was developed and used with a portable OCT system to image in the surgical suite and pathology laboratory. The imaging probe has a 2 cm working distance which allows the surgeon to maneuver the beam within the tight confines of the surgical field. Preliminary results demonstrate the feasibility of intraoperative OCT imaging in human subjects as well as imaging in a pathology laboratory environment.