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Zero-mode waveguides for single molecule analysis and fast DNA sequencing

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5 Author(s)
Levene, M. ; Dept. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA ; Korlach, J. ; Turner, S. ; Craighead, H.G.
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Summary form only given. Zero-mode waveguides enable the observation of which fluorescent nucleotide analog is being added to the double stranded product in the active site of the enzyme while excluding signal from freely diffusing fluorescent species. The effectiveness of zero-mode waveguides is demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside zero-mode waveguides, using micromolar concentrations of fluorescently labeled nucleotide analogs.

Published in:

Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the

Date of Conference:

24-24 May 2002