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This study was designed to evaluate the EM effects of mobile phone on rats' brain. 23 female Wistar Albino rats weighing 120-150 g were divided into two groups. In the first group, test animals that consist of 9 rats were exposed to mobile phone 2×30 min/day during four weeks. The specific gravity values of rats' brain were assessed in 5 of them and the blood-brain barrier activity were tested in 4 of them. The results were compared with control animals that they were kept in the same condition without exposing to radiation. It was not observed any apparent difference between control and test group results. The second group that consists of 6 rats was used to investigate the temperature effect of mobile phones on brain. They were exposed by mobile phone and helix antenna fed by 900 MHz, 2 W, RF signal generator for 6 min. The temperature variation was recorded by fluoroptic thermo probes in the subcutane and 2 mm deep under duramater at the upper part of the ear. After each exposure, 5 min intervals were applied to provide thermal equilibrium in brain tissue. The maximum temperature increases is 0.31°C in the brain and 0.93°C in the subcutane. SAR values were obtained from the measured temperatures for different EM fields.