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Scanning Transmission X-ray Microscopes are used to image 0.1-10 micron thick specimens at 20-50 nm resolution using zone plate focusing. Many of the applications of these instruments make use of the elemental and chemical sensitivity of XANES spectroscopy combined with microscopy. Recent instrumentation developments are described, including cryoSTXM for frozen hydrated specimens, tomography, configured detector for multiple contrast recording, etc. Diffraction-based imaging is being developed for high resolution 3D microscopy.