By Topic

Decomposition of field-induced transmembrane potential responses of single cardiac cells

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Sharma, V. ; Cardiac Rhythm Manage., Medtronic Inc., Minneapolis, MN, USA ; Lu, S.N. ; Tung, L.

In this study, we used a multi-site optical mapping system to record field-induced responses of single cells isolated from guinea pig hearts. The cells were stained with voltage sensitive dye di-8-ANEPPS and stimulated with two uniform field (S1-S2) pulses along their longitudinal axes. The first pulse (S1=5 ms, <10 V/cm) was applied during rest and elicited an action potential. The second pulse (S2=10 ms, 4-50 V/cm) was applied 15 ms after the break of the S1 pulse (during the action potential plateau). The transmembrane potential responses, V m Fs, were optically recorded from up to 12 sites along the cell length using a fiber optic based optical mapping system at a resolution of 17 or 25 μm. The field-induced V m Fs had a complex spatio-temporal pattern. We show that these responses can be decomposed into simpler components. The first component, termed the differential-mode component (V md F), is like the response of a passive cell. The second component, termed the common-mode component (V mc F), is identical all along the cell and adds a constant offset to the differential mode response of various sites along the cell length, to produce the total V m F responses of the cell.

Published in:

Biomedical Engineering, IEEE Transactions on  (Volume:49 ,  Issue: 9 )