By Topic

New methods of DNA oligomer - binding and detection in the DNA microarray technology

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

1 Author(s)
A. Gyorffy ; Dept. of Electron. Technol., Budapest Univ. of Technol. & Econ., Hungary

Widely used molecular-chips of today are expression type DNA-microarrays. These chips are not commonly used in clinical practice due to the high cost, the need for extensive laboratory devices and due to not well clarified results. Therefore the need for simple mass-production methods is constantly growing. In our work we have developed a new DNA immobilisation method for DNA binding on polypyrrole modified gold electrode. After immobilisation of biotin labeled DNA on electrode the detection was performed through streptavidin-texas red dye binding. The unlabeled DNA probes were detected using bis benzimide dye. For the visualisation of the DNA in detection we used confocal fluorescence microscope. We investigated the influence of the polymerisation time to thickness and quality of the polymer film and the influence of the quantity of DNA for detection and immobilisation. In our conception we targeted to build a clinically applicable chip, to be used for the identification of PCR amplified DNAs for known mutations of inherited diseases in the future.

Published in:

Polymers and Adhesives in Microelectronics and Photonics, 2002. POLYTRONIC 2002. 2nd International IEEE Conference on

Date of Conference: