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In this paper, we propose a method of low-noise signal readout using frame oversampling and a CMOS image sensor with non-destructive high-speed readout mode. The technique enables the use of high-gain column amplifiers and the digital integration of signals without noise accumulation. The column amplifier is effective for reducing the noises due to the wideband amplifier and the quantization noises. Simulation results show that the noise can be reduced by a factor of 20 log10 M [dB] where M is the oversampling ratio.