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Developing culture-based biochips for detecting microorganisms in wastewater treatment

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6 Author(s)
H. B. Eluru ; Dept. of Electr. & Comput. Eng. & Comput. Sci., Cincinnati Univ., OH, USA ; A. Polaczyk ; R. Chhabra ; B. Kinkle
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In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophilic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed in order to fabricate such biochips. The developed technique uses butanol solvent to keep paraffin liquid during deposition by spin-coating and thick photoresist for patterning. Using this approach, 18 mm glass biochips with paraffin layers of 5-70 μm have been successfully fabricated. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment

Published in:

Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on

Date of Conference:

2002