By Topic

Design and testing of an underwater microscope and image processing system for the study of zooplankton distribution

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Akiba, T. ; Life Electron. Res. Center, Agency of Ind. Sci. & Technol., Ibaraki, Japan ; Kakui, Y.

A method that can monitor the density of zooplankton at an adequate spatio-temporal resolution is desired in oceanic ecosystem research. To address this need, we have developed a submersible microscope equipped with a noninterlace CCD camera. The target plankton for this microscope includes Copepoda, Ploima, and Ciliata, which are dominant species in the coastal waters around Japan. In addition, the requirements of systems for underwater imaging of zooplankton are discussed. The key issues investigated for their possible influence on system performance are lens selection, camera selection, and method of illumination. Higher order local autocorrelational (HLAC) masks are used to extract features from images. Combining these features with multivariate analysis, which is a two-step feature extraction method, results in a powerful tool for extracting general information from images. In our procedures, a set of these features provides a 33-dimensional vector. To identify and count zooplankton, canonical correlation analysis and discrimination analysis are performed. This allows zooplankton to be counted and classified into taxonomic units. Another canonical correlation analysis was made for the sizing of the plankton. Proof of the principle experiment is obtained with images of both preserved and living Copepoda.

Published in:

Oceanic Engineering, IEEE Journal of  (Volume:25 ,  Issue: 1 )