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In this letter, we report on measurements of carbon nanotube (CNT) field-effect transistors with high on/off ratio to be used as nonvolatile memory cells operating at room temperature. Thousands of memory devices have been realized using a complete in situ fabrication method. The self-aligned fabrication process allows large-scale production of CNT memory devices with high yield. The memory function is obtained by the threshold voltage shift due to the highly reproducible hysteresis in the transfer characteristics. The ratio of the current levels between a logical ldquo1rdquo and a ldquo0rdquo is about 106.