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Optical Separation of Cells on Potential Energy Landscapes: Enhancement With Dielectric Tagging

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10 Author(s)

We review the emergent techniques of microfluidic sorting of colloidal and cellular samples using optical forces. We distinguish between what we term as passive and active forms of particle sorting where we can sort either with the use of a fluorescent marker (active) or based on physical attributes alone (passive). We then examine cell sorting with optical potential landscapes such as a Bessel light beam and a multibeam interference pattern. For both forms of optical potential energy landscape, we further present the possibility of enhancing the optical sorting process by tagging dielectric microspheres onto the cells. The results suggest that the methodology of tagging can enhance the sorting of cells as they subsequently respond more strongly to an applied optical field or potential energy landscape. This technique presents a simple method to enhance the sorting process.

Published in:

Selected Topics in Quantum Electronics, IEEE Journal of  (Volume:13 ,  Issue: 6 )

Date of Publication:

Nov.-dec. 2007

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