By Topic

A Spectrofluorometer for Recording of Intracellular Oxidation-Reduction States

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
B. Chance ; Department of Biophysics, University of Pennsylvania, Philadelphia, Pa. ; V. Legallais

A simple and versatile microspectrofluorometer is described together with suitable electronic circuits for single-differential-or double-aperture operation. The apparatus is suitable for measuring oxidation-reduction states of mitochondrial and cytoplasmic pyridine nucleotide in various organs with apertures ranging from 15 microns up to a few millimeters. Suitable optical and electronic components are briefly described together with some of their limitations. The application of the method to the state of oxygenation of brain and liver together with the recording of hormonal responses in the heart is indicated. An outline of the limitations of the present apparatus and desirable future developments is included.

Published in:

IEEE Transactions on Bio-medical Electronics  (Volume:10 ,  Issue: 2 )