By Topic

On-Chip Capacitance Sensing for Cell Monitoring Applications

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Somashekar Bangalore Prakash ; Dept. of Electr. & Comput. Eng., Maryland Univ., College Park, MD ; Pamela Abshire

We describe an integrated circuit for sensing the substrate coupling capacitance of anchorage-dependent living cells in a standard culture environment. Capacitance is measured using charge redistribution in response to weak, low frequency electric field excitations. The underlying biophysical phenomenon results primarily from the insulating nature of the cell structure and the counterionic polarization in the surrounding aqueous medium. The measured capacitance depends on a variety of factors related to the cell, its growth environment and the supporting substrate. These include membrane integrity, morphology, extracellular ionic concentration, adhesion strength, and substrate proximity. The measured capacitance is an indication of both the interaction between cells and substrate and cell health. The capacitance sensor uses the principle of charge sharing and translates sensed capacitance values to output voltages. The sensor chip has been fabricated in a commercially available 0.5-mum, 2-poly 3-metal CMOS technology. The sensing technique does not require direct electrical connection to the aqueous culture medium. We report results from experiments demonstrating on-chip tracking of cell adhesion as well as long term monitoring of cell viability in vitro

Published in:

IEEE Sensors Journal  (Volume:7 ,  Issue: 3 )