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This paper presents an agent-based software environment for testing Web-based applications. The infrastructure of the system consists of a light weight agent platform that supports agent communication, an ontology of software testing that enables flexible integration of multiple agents, and a formalism using XML to represent both the basic and compound concepts of the ontology. Relations between testing concepts are defined and their properties are analysed. A number of agents are implemented to perform various tasks in testing Web-based applications. Broker agents use the ontology as a means of inferences to manage the knowledge about agents and assign each task to the most appropriate agent.