Simple and rapid connection of chicken embryonic cardiovascular system

We propose a simple and rapid microcardiovascular connection method called suction-induced vascular fixation (SVF) method for the achievement of bionic simulator with using chicken embryonic heart. The advantages of proposed method with using a microfluidic device are as follows: (1) operation of flexible objects (blood vessels), (2) alignment the blood vessels concurrently, and (3) reduction the DOF of the blood vessels. From the experimental results, we confirmed that four cardiovascular can be induced into the fabricated device concurrently. Furthermore, we have succeeded in connecting to a heart of chick embryo with microtubes. We have also succeeded in construction of hybrid circulatory system between artificial tubes and blood vessels led to the heart of chick embryo.