Fabrication and characterization of nano-structured conducting polymer electrodes for glucose biosensor applications

This work describes fabrication and characterization of a novel nano-porous polypyrrole (PPy) electrode and its application in amperometric biosensors. Nano-porosity was artificially introduced into PPy films by using Pt sputtered nano-porous Alumina discs as base electrodes during polymerization. The resulting nano-structured PPy has helped increasing the performances of biosensors by increased effective surface area for detection of electron flow. When GOx was immobilized by physical adsorption, the sensor showed much stability against H
₂
O
₂
and enhanced characteristics for glucose sensing. This sensor resulted in rapid, simple, and accurate measurements with high sensitivities (3.7 AM
^-1
cm
^-2
and 3 mAM
^-1
cm
^-2
for H
₂
O
₂
and glucose respectively) and very fast response times (3 s in each case).