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A sensitive analytical method for triclosan (TCS) in water samples, which involves enrichment with bamboo-activated charcoal (BAC) and detection with HPLC-ESI-MS is developed. The effects of some important operation parameters, such as the eluant and its volume, flow rate, the volume and the acidity of the sample and the amount of bamboo-activated charcoal, on the recovery of TCS were investigated and optimized. Under the optimum conditions, the spiked recoveries of TCS in real water samples were achieved in the range of 108.5%-113.4%. The linearity of the method was observed in the range of 0.02-20 μg L-1, with correlation coefficients (r2) >; 0.9990. The limit of detection ( LOD ) was 0.002 μg L-1 based on the ratio of chromatographic signal to base line noise(S/N = 3). The concentrations of TCS in the real environmental water samples collected in Darning Lake, Xiaoqing River and Yellow River at Jinan, Shandong Province were also determined using this method.