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Effect of the extract of Fructus tribuli (EFT) on growth of Bifidobacterium bifidum BB01 and BB03 was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could improve the buffer ability of MRS and promote the growth of Bifidobacterium bifidum BB01 and BB03. The optimum concentration of EFT in MRS media was 5% at incubation 18h for Bifidobacterium bifidum BB01 and 3% at incubation 24h for Bifidobacterium bifidum BB03.