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A bacterium(strain yc02) was isolated from environment, which could degrade polyvinyl alcohol. It was preliminary identified as the genus of Pseudomonas sp. The further degrading characterization research showed that the optimal degradation temperature, culture time, rotary speed and the initial pH of fermentation medium were 25°C, 60 h, 180 rpm and 7.5 respectively, the ratio of degradation reached 95.56% under this conditions.