Regular, almost quadratic pits were created in an island of C60 molecules on a rutile TiO2 (110) surface using noncontact atomic force microscopy at room temperature. Upon gradually approaching the scanning tip toward the surface, the interaction between the tip and the C60 island was increased until manipulation was achieved. Analyzing the manipulation process unambiguously revealed that the manipulation was performed in the repulsive regime. Retracting the tip allowed for reproducible imaging the C60 island after the manipulation process. Moreover, whole islands could be reshaped or even removed when scanning with appropriate scanning parameters.