We present a demonstrating Web application for biomedical imaging and visualization, addressing its capability of implementing comprehensive image processing algorithms at the server side where proprietary datasets reside and direct access to them by client-side applications is restricted. The main technical challenge is to make the functionality of the dedicated imaging and visualization programs (usually compiled from a native programming language such as C++) publicly available via a Web based interface. Accessing information of the original image data and algorithmic details should be hidden, thus scripts executed by the webpage browser should be avoided. In this regard, we propose a solution based on the ASP.NET framework. Managed codes in C# are developed for Web controls, server-side GUI and relational database management; whereas unmanaged server-dependent imaging programs are invoked from within the managed programs. The advantages of such a Web application include (a) high security of the proprietary data which is always a primary consideration in deployment of a networked system, (b) thin-client strategy in which heavy computing tasks are completed on the dedicated server with high-performance hardware, and (c) less demand for network bandwidth as only the rendering results instead of multiple high-dimensional datasets need to be transferred. The proposed approach is believed to be beneficial for building the Web-based Laboratory Information System (LIS) and Radiology Information System (RIS).