Promoter fragment of alkaline protease gene was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR. The fragment was sequenced and analyzed, then submitted to GenBank (EU130686). The results showed that it contained several typical promoter charactered regions and two reverse translation frames located in - 538 to - 370 bp and - 275 to - 128 bp region. Deletion analysis of the sequence indicated that 414 bp upstream of the TSS exhibited predominant promoter activity, while an 105 bp length could serve as this function. Additionally, our data demonstrated that a representative Sec- type signal peptide structure presented in PB92 alkaline protease signal peptide. The efficiency of PaprE- AprE signal peptide gene cassette was validated by its driving a plant sweet protein monellin gene highly expression in Bacillus subtilis 1A751.