The treatment of the rupture tendon is still an obstacle in respect of suture method. Therefore, such shortcoming in repairing fracture tendons could be overcome through tissue engineering approaches. The aim of present study is to investigate the influence of processing parameters on PLGA scaffold morphology and fiber diameters and the effect of the tendon fibroblast with the obtained novel tissue engineering electrospun-PLGA. The nanofiber scaffold was produced via electrospinning. The result shows that the nanofiber diameter and morphology could be controlled under different processing parameters. The Achilles tendon fibroblasts exhibit enough cell viability and were attached with spindle shape on the PLGA nanofiber scaffold. Therefore, PLGA nanofiber scaffold have the potential for Achilles tendon repair.