A fuzzy logic approach, called FuzzyTRN, to infer transcriptional regulatory networks (TRN) in Saccharomycescerevisiae is proposed. FuzzyTRN predicts potential regulators and their target genes using sequences analysis on transcription factor binding sites (TFBS) of transcriptional factors (TF) and promoter region of target genes. Those potential regulators and target genes are used to form vertices in the TRN. Furthermore, multiple sets of microarray gene expression data (MGED) are used by FuzzyTRN to predict links in the TRN. FuzzyTRN predicts transcriptional interactions by recognizing expression patterns of genes. In this study, a number of confirmed genetic interactions are utilized to train FuzzyTRN. 112 indirect genetic interactions that were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) experiments, and 259 and 86 direct genetic interactions that were collected by TRANSFAC database and literature surveying, were used as training set in this work. A simulation that encompasses 170 TFs and 40 target genes has been conducted and checked against YEASTRACT database to evaluate the performance of the proposed algorithm.