This paper presents a framework for agent-enabled semantic integration in the design of distributed digital museum. In this framework, Web Services provides remote information process services which can be discovered and invoked by middle-layer agent to satisfy multiple needs. Domain Ontology is used to characterize and model the semantics that are embedded in the digital museum. Concept-mapping Agent is deployed to set up the semantic relationships between Domain Ontology and relational databases and feedback to middle-layer agent, in doing so, we can get directly benefit from middle-layer agent. During the user request answer process, middle-layer agent can dynamically and quickly discovered the services, executed the remote calling of services, combined the results and finally feedback to user. Furthermore, the paper gave out a Digital Museum Information Retrieval System (DMIRS) , which is designed, based on this framework and demonstrated to be stable and efficient.