In this paper, a control structure that makes possible the integration of a kinematic controller and an adaptive fuzzy controller for trajectory tracking is developed for nonholonomic mobile robots. The system uncertainty, which includes mobile robot parameter variation and unknown nonlinearities, is estimated by a fuzzy logic system (FLS). The proposed adaptive controller structure represents an amalgamation of nonlinear processing elements and the theory of function approximation using FLS. The real-time control of mobile robots is achieved through the online tuning of FLS parameters. The system stability and the convergence of tracking errors are proved using the Lyapunov stability theory. Computer simulations are presented which confirm the effectiveness of the proposed tracking control law. The efficacy of the proposed control law is tested experimentally by a differentially driven mobile robot. Both simulation and results are described in detail.