Metalloproteins thin films

The structure and orientation of metalloproteins adsorbed onto semiconductor and metal surfaces were investigated. X-ray photoelectron spectroscopy (XPS) techniques were used in conjunction with novel myoglobin derivatives. In these myoglobin derivatives, a pentammine ruthenium (III) group is attached to specific histidine residues on the protein surface. The Fe-Ru distance in these derivatives was calculated and found to be on the order of 13-25 AA. Measurements of the intensity ratio of the Ru 3p/sub 3/2/ or Ru 3d/sub 5/2/ to Fe 2p/sub 3/2/ XPS peaks provide an indicator of the degree of ordering in the film.<
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