LED Lighting Source Design for Fluorescent Microscope

In this study, we design a LED light source system for a fluorescent microscope used for observation of cells with specific fluorescent molecules. The required number of LED light sources have been evaluated first. Then we use the 3D graphics software and the light tracing simulation software to construct the LED light source model. A collimating lens array, a confocal system, and a diffuser are combined with the LED light source system, can produce a uniform light distribution and small beam-spread angle. After analysis, the center illumination on the projection area of this system can reach 13.2 W/m2, the optical efficiency can reach 72.33%, and the uniformity can reach 1.32. Finally, we also analyzed the tolerance of the system on the installation. The tolerance of the displacement along the x-axis and the z-axis of the light source installed in the space are respectively about 0.4mm and 0.3mm.