Single grid image based calibration of an optical microscope

In this study, we propose a practical method, in which a single grid image is utilized, to realize the calibration of an optical microscopic. This method mainly comprises four steps, including 1) Automatic detection of line features and point features from the grid image; 2) Correction of the lens Distortion utilizing the straightness of the line feature; 3) Estimation of the homograph using the corrected lines and points; 4) Solving the full intrinsic and extrinsic parameters of the microscope. Experimental results show that the proposed method is effective and reliable for the calibrating of the microscope with quite small field of view.