Microfluidic delivery of genome-editting materials into iPS-cardiomyocytes using synergistic electroporation and shear stress

Genetic heart diseases are challenging to treat since the mutations occur in all cells. The gene-editing technique, CRISPR allows specific correction of the genome. This paper reports a microfluidic technique using both electroporation and mechanical shear stress to create pores complementarily along different poles on the cell membrane to deliver CRISPR genome-editing components into iPS (induced pluripotent stem cells) - cardiomyocytes. A transfection efficiency of 11.2% was achieved in the preliminary trials.