Improved patch-clamp amplifier using software-based high-frequency boost

The large feedback resistors in the headstage of the patch-clamp amplifier lower the recording bandwidth to less than 100 Hz. A software-based high-frequency boost (SHB) was introduced in this paper. The algorithm consisted of two parts: 1) headstage dynamic characteristic identification using 4SID method; 2) bandwidth compensation using zero-pole matching. Both time and frequency domain analysis demonstrated SHB should be a satisfied alternative to the traditional high-frequency boost circuit.