Plasmatreatment induces blood clot formation; protein aggregation and hemolysis

Surgical site bleedings of spurting and oozing, have been controlled by clipping and ligation, and using surgical hemostats and energy devices, respectively. Notably, the intended use of the energy devices and surgical hemostat are to obtain greater visibility rather than to prevent excessive blood loss. It was pointed out that low temperature plasma (LTP) treatment could be used the same as surgical hemostats, because it could promote blood clot formation by stimulation of the both platelet aggregation and activation of coagulation cascades. While neither hemolysis nor serum proteins aggregation in these earlier studies, LTP treatment with our devices could form clot with hemolysis from the isolated erythrocytes by use of density gradient, and make aggregations of materials of serum proteins1. The formed clots were histologically fibrous membrane-like structure, and electron microscopically monotonous material with high electron density. Thus, our results and those of earlier studies figured out two types of blood clot formation by LTP treatment. An essential point is whether the effect of LTP was largely confined to blood coagulation system or extend to serum protein aggregations or hemolysis. Based on these findings, we will discuss the feasible to use the blood clot formation induced by LTP treatment in clinical practices.