Methylene blue-containing nanoliposomes biological distribution and release after dual frequency sonication

In this study, a technique to overcome the limitation of systemic administration of Methylene (MB), encapsulation of methylene blue in polymeric liposomes and drug release after dual frequency sonication has been investigated. After loading methylene blue into liposomes of the mice, the drug encapsulation efficiency and stability is measured by fluorometrically and its biodistribution of drug is studied. The level of drug release in vitro is checked using single and dual frequency sonication (1MHz+40kHz) with intensity through fluorometrically. The dynamic light scattering (DLS) studies showed that the synthesized liposomes had an average size of 66±5 nm. The results indicate that a 5 min applying the dual frequency sonication causes a MB release of about 79% nanoparticles. The biodistribution study revealed that, the drug content in the group that received liposomal drugs in the tumor tissue was significantly higher than in the group receiving methylene blue in its free form (p<;0.05). Also, for the group that received liposomal MB, the ultrasound measurement showed significantly higher drug content in the tumor tissue than other groups (p<;0.05). It is concluded that the sonication with a dual frequency system causes the drug to release from the liposome and increases drug uptake ability.